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Global control of cell-cycle transcription by coupled CDK and network oscillators

TitleGlobal control of cell-cycle transcription by coupled CDK and network oscillators
Publication TypeJournal Article
Year of Publication2008
AuthorsOrlando DA, Lin CY, Bernard A, Wang JY, Socolar JES, Iversen ES, Hartemink AJ, Haase SB
Date Published2008 Jun 12
KeywordsBiological Clocks, Cell Cycle, Cyclin-Dependent Kinases, Cyclins, G1 Phase, Gene Expression Regulation, Fungal, Mutation, Periodicity, S Phase, Saccharomyces cerevisiae, Time Factors, Transcription, Genetic

A significant fraction of the Saccharomyces cerevisiae genome is transcribed periodically during the cell division cycle, indicating that properly timed gene expression is important for regulating cell-cycle events. Genomic analyses of the localization and expression dynamics of transcription factors suggest that a network of sequentially expressed transcription factors could control the temporal programme of transcription during the cell cycle. However, directed studies interrogating small numbers of genes indicate that their periodic transcription is governed by the activity of cyclin-dependent kinases (CDKs). To determine the extent to which the global cell-cycle transcription programme is controlled by cyclin-CDK complexes, we examined genome-wide transcription dynamics in budding yeast mutant cells that do not express S-phase and mitotic cyclins. Here we show that a significant fraction of periodic genes are aberrantly expressed in the cyclin mutant. Although cells lacking cyclins are blocked at the G1/S border, nearly 70% of periodic genes continued to be expressed periodically and on schedule. Our findings reveal that although CDKs have a function in the regulation of cell-cycle transcription, they are not solely responsible for establishing the global periodic transcription programme. We propose that periodic transcription is an emergent property of a transcription factor network that can function as a cell-cycle oscillator independently of, and in tandem with, the CDK oscillator.

Alternate JournalNature

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