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Rasp, a putative transmembrane acyltransferase, is required for Hedgehog signaling.

TitleRasp, a putative transmembrane acyltransferase, is required for Hedgehog signaling.
Publication TypeJournal Article
Year of Publication2002
AuthorsMicchelli CA, The I, Selva E, Mogila V, Perrimon N
JournalDevelopment (Cambridge, England)
Date Published2002 Feb
KeywordsAcyltransferases, Amino Acid Sequence, Animals, Drosophila melanogaster, Drosophila Proteins, Hedgehog Proteins, Membrane Proteins, Molecular Sequence Data, Phenotype, Proto-Oncogene Proteins, Signal Transduction, Transcriptional Activation, Wnt1 Protein

Members of the Hedgehog (Hh) family encode secreted molecules that act as potent organizers during vertebrate and invertebrate development. Post-translational modification regulates both the range and efficacy of Hh protein. One such modification is the acylation of the N-terminal cysteine of Hh. In a screen for zygotic lethal mutations associated with maternal effects, we have identified rasp, a novel Drosophila segment polarity gene. Analysis of the rasp mutant phenotype, in both the embryo and wing imaginal disc demonstrates that rasp does not disrupt Wnt/Wingless signaling but is specifically required for Hh signaling. The requirement of rasp is restricted only to those cells that produce Hh; hh transcription, protein levels and distribution are not affected by the loss of rasp. Molecular analysis reveals that rasp encodes a multipass transmembrane protein that has homology to a family of membrane bound O-acyl transferases. Our results suggest that Rasp-dependent acylation is necessary to generate a fully active Hh protein.

Alternate JournalDevelopment

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